ANALYSIS OF CANDIDATE GENES AND THEIR FUNCTIONS BASED ON QTLS ASSOCIATED WITH ABIOTIC STRESS TOLERANCE IN GOSSYPIUM HIRSUTUM L. VARIETIES

Authors

  • Kurbanov Abrorzhon Yorkinovich1 Doctor of Agricultural Science, Senior Researcher of Cotton Breeding, Seed Production and Agrotechnologies Research Institute1 Author
  • Mamedova Feruza Fakhriddinovna1 Doctor of Philosophy in Agricultural Science of Cotton Breeding, Seed Production and Agrotechnologies Research Institute1 Author
  • Boboyev Sayfulla G‘ofurovich 2 DSc. (Biol. Sci.), Professor of Tashkent State Agrarian University Author
  • Djumayev Sanjar Shukurovich1 junior researcher of Cotton Breeding, Seed Production and Agrotechnologies Research Institute1 Author
  • Isayeva Rayxona Madaminjon qizi3 Master student of National University of Uzbekistan named after Mirzo Ulugbek Author

Keywords:

Gossypium hirsutum, Drought stress, QTL mapping, Candidate genes, Transcription factors, GhWRKY70, GhDREB2, CRISPR/Cas9, Genetic selection, Wild cotton germplasm (G. tomentosum)

Abstract

In this study, we identified quantitative trait loci (QTL) associated with drought‑tolerance traits in Gossypium hirsutum L. and performed functional evaluation of candidate genes within these QTL regions. Specifically, genes for transcription factors (GhWRKY27, GhWRKY70, GhDREB2) and other stress‑responsive genes (GhCAT1, GhCIPK6, SnRK2.6) were analyzed in QTL located on chromosomes D01, A05, A11, and D08. The presence of ABRE elements in promoters, consistency with previous transcriptomic data, and detected epistatic interactions support the involvement of these genes in drought stress tolerance. Additionally, novel QTL regions on chromosomes A12 and D05 were identified for the first time and confirmed to be associated with tolerance traits. Analysis revealed that most drought‑enhancing alleles originated from the wild G. tomentosum genotype. These candidate genes are promising targets for marker-assisted selection, genome editing (CRISPR/Cas9), and transgenic studies. The findings contribute to the genetic understanding necessary for developing drought‑tolerant cotton cultivars.

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Published

2025-09-03

Issue

Vol. 2 No. 3 (2025): INTERNATIONAL CONFERENCE ON SCIENCE, ENGINEERING AND TECHNOLOGY

Section

Articles

How to Cite

ANALYSIS OF CANDIDATE GENES AND THEIR FUNCTIONS BASED ON QTLS ASSOCIATED WITH ABIOTIC STRESS TOLERANCE IN GOSSYPIUM HIRSUTUM L. VARIETIES. (2025). INTERNATIONAL CONFERENCE ON SCIENCE, ENGINEERING AND TECHNOLOGY, 2(3), 3-7. https://eoconf.com/index.php/icset/article/view/88